Resources

Helpful Guides to Get Started

So, I would suggest you browse through the following links to obtain some more structured ideas of hwo you're going to layout the cloning process ahead of you. To start, go to Addgene, SnapGene, and Goldbio below, all are amazing resources and are beginner friendly.

Addgene resources:

• Addgene's Plasmid 101 blog
• This has almost everything I covered in this blog and has so much more. If anything I said didn't make sense to you, check out their blog and see if that clears anything up. 
• Commonly used protocols
• This goes into basic lab bench work (PCRs, RE, plate pouring, etc) but also goes into the cloning techniques themselves, some I didn't even cover!

SnapGene Resources:

• SnapGene Academy to learn cloning
• This is similar to the Addgene Plasmid blog, it is a resource to learn the concepts behind the process as well.
• SnapGene Cloning Cycle
• Same here, this resource has some tips for getting started and shows a flowchart even more general than mine!

Zymo Research Resources:

• Crash Course: Plasmid DNA Fundamentals
  
• This resource talks about a plasmid's basic components as well as ORIs and copy number background. This resource should be viewed before looking into the cloning concepts if you're new to plasmids

Goldbio Resources:

• An introduction to cloning
• If 

NEB Resources:

• Overview, history and types of cloning

NCBI:

• Good for BLASTing sequences

Phtyozome:

• Good source for plant genome BLASTing

Plasmid Vector Repositories:

This list is thanks to Dr. Aimee Malzahn, the project coordinator for PlantGene. 

• Addgene
• DNASU
• MolecularCloud
  
• Standard European Vector Architecture (SEVA) 4.0
• Arabidopsis Biological Resource Center
• Belgian Coordinated Collection of Microorganisms/GeneCorner
  
• VIB-UGENT
  
• iGEM
  
• European Plasmid Repository

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Jargon and Concepts

Cloning

Overview

1. In Silico

2. Molecular

2. E coli

4. Agrobacterium

5. Plant