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This site is to be supplemental material to my presentation at SIVB in June of 2024 and 2025. These resources will be available long after my talks for you to go back and visit.

The target audience is those who are in or have completed a graduate degree in plant sciences. The purpose of this presentation is to supply researchers with the tools to start cloning for plant transformation in their labs. 

Please follow navigate to each of the talk's sections using the links below:

Video Presentation of Cloning and Vector Construction Basics 

Use these links to go to the various modules for this talk!

Jargon and Concepts

Cloning

Overview

In Silico Cloning 

Molecular Aspects of Cloning

E coli Aspects of Cloning

 Agrobacterium in Terms of Cloning 

 Plant Transformation

Resources

See the links on the left(desktop) or below(mobile) to go to the various modules for this talk!

Modular Cloning Jargon and Concepts

Restriction Enzymes for Modular Cloning

GoldenGate Overview

GoldenGate Options for Plant Science

Setup and Staying Organized

Misc. Notes

I owe a great thanks to PlantGene who supported me with resources and offered me the opportunity to present at SIVB. 

Who am I? 

My name is Katie Toomey, and I am a PhD student in the Parrott lab at UGA. I am researching prime editing for precision gene modifications and the development/utilization of landing pads for targeted genome insertion. I love science communication and helping others learn the tools they need to succeed.